THE EXTRACTION AND PURIFICATION OF POLYPHENOL OXIDASE IN WHITE YAM - 2021 Final Year Project Research Topics And Materials
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TABLE OF CONTENT

Contents pages

Title page ..i

Approval page.ii

Dedication.iii

Acknowledgementiv

List of tables and figures.v

Table of contents…vi

Abstractvii

Chapter one

1.1 Introduction. 1

1.2 Historical features.1

1.3 Nutrient composition of yam…1

1.4 Polyphenol oxidase enzymes3

Chapter two

Literature review5

Yam diseases.5

Storage diseases6

Growth and harvest of yam tubers….6

Yam crop productionstages and process of yam production8

Flavonoids10

Functions of flavonoids..10

Tannins11

Biosynthesis and analysis of phenol..12

Biosynthesis of phenol12

Test for phenol..,,13

Uses of phenol.13

Properties of phenol14

Enzymes ……14

Types of enzymes 14

An overview of polyphenol oxidase enzymes.16

CHAPTER THREE

Experimental instrumentation and Materials..18

Flowchart for polyphenol oxidase preparation of… 19 partial purification from white yam…19

Methodology :isolation and purification of enzyme23

Crude enzyme preparation ..23

Acetone precipitation 24

Ammonium sulphate fraction..24

Dialysis ..25

Centrifugation ..26

Sephadex gelfilteration 26

CHAPTER FOUR

Result 127

Definition..27

Result 2..30

PH optimum..34

CHAPTER FIVE

Discussion35

Conclusion …35

Recommendation…..36

Reference..38

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THE EXTRACTION AND PURIFICATION OF POLYPHENOL OXIDASE IN WHITE YAM

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