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The identification of prevalence microbes associated with frozen fish marketed in owerri metropolis

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Abstract

This research work is on The identification of prevalence microbes associated with frozen fish marketed in owerri metropolis. Aquaculture products can harbour pathogenic bacteria, which are of the natural micro-flora of the environment. An in-vitro assay was carried out to ascertain and identify major bacterial contaminants of frozen fish, which had constituted an important dietary intake of the people of Owerri, Nigeria. Fish samples collected were identified and bacterial load of the samples determined using pour plate method. Differentiations and characterizations of various isolates were based on biochemical reactions and gram-staining technique. Frozen Sardine, Mackerel, and Croaker fish were used for the study. In-vitro assay result revealed that the samples were predominantly contaminated by three bacteria species viz: Staphylococcus aureus, Escherichia coli and Pseudomonas specie. Incidences of the various isolates in the culture were found to be 45%, 33% and 22%, respectively. The mean bacteria load of the isolates was1.33x109CFU g-. This value was found to be markedly higher than the recommended public health and safety standard value of between 5.0 x 105 CFU/ g-, approved by Nigerian National Agency for Drugs Administration and Control (NAFDAC).

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