Formulation of microbial growth medium from sweet potato

Description

Formulation of microbial growth medium from sweet potato.

Abstract

Agar medium preparation from sweet potato without dextrose was evaluated as a growth medium for two phytopathogenic fungi. Sabouraud dextrose agar (S.D.A) was used as a control. Sweet potato medium as well as sabouraud dextrose agar bread and garri. Culture was incubated for 3days, after which growth of micro-organism was observed. Lactophenol stain was done to dictate the particular micro-organism that grew on the plate. Aspergillum was observed on the plate that contained Garry, and mould (penicellium) was observed on the plate that contained bread, on both sabouraud dextrose agar and sweet potato extract medium. There was greater growth on sweet potato extract medium while on sabouraud dextrose agar. Less growth appeared because of streptomycin that was incorporated to inhibit some growth of micro-organisms on both. Total plater count shown was 1.24×106 cfu/ml from bread on sabouraud dextrose agar (S.D.A). 7.6X10 SCFU./ML form Garry on sabouraud dextrose agar, 9.4×105 cfu/ml. From Garry on sweet potato extracted medium and 1.74×106 cfu/ml. From bread on sweet potato extract medium.